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基本信息 載體類型: 植物載體,農(nóng)桿菌雙元表達(dá)載體 載體抗性: Kan 載體標(biāo)簽: 3×FLAG 表達(dá)水平: 高拷貝 載體大小: 10511bp 啟動(dòng)子: Lac 克隆方法: 多克隆位點(diǎn),限制性內(nèi)切酶 5' 測(cè)序引物: 35S primer: 5’-CTATCCTTCGCAAGACCCTTC-3’ 3' 測(cè)序引物: / 備注: 如有侵權(quán)請(qǐng)產(chǎn)權(quán)持有人盡快聯(lián)系法務(wù)15927229196
質(zhì)粒圖譜和多克隆位點(diǎn)信息
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載體描述
pCAMBIA1300-35S-3XFlag在pCAMBIA1300-35S載體的基礎(chǔ)上改造而來,植物融合表達(dá)載體。
載體序列
ORIGIN
1 gaattcggtc cccagattag ccttttcaat ttcagaaaga atgctaaccc acagatggtt
61 agagaggctt acgcagcagg tctcatcaag acgatctacc cgagcaataa tctccaggaa
121 atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taactgcatc
181 aagaacacag agaaagatat atttctcaag atcagaagta ctattccagt atggacgatt
241 caaggcttgc ttcacaaacc aaggcaagta atagagattg gagtctctaa aaaggtagtt
301 cccactgaat caaaggccat ggagtcaaag attcaaatag aggacctaac agaactcgcc
361 gtaaagactg gcgaacagtt catacagagt ctcttacgac tcaatgacaa gaagaaaatc
421 ttcgtcaaca tggtggagca cgacacactt gtctactcca aaaatatcaa agatacagtc
481 tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg aaacctcctc
541 ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc
601 tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac
661 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca
721 accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca
781 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag
841 agaacacggg ggacgagctc ggtacccggg gatcctctag agtcgacatg gactacaaag
901 accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat gacaagtaac
961 tgcagagctt tcgttcgtat catcggtttc gacaacgttc gtcaagttca atgcatcagt
1021 ttcattgcgc acacaccaga atcctactga gttcgagtat tatggcattg ggaaacatgt
1081 ttttcttgta ccatttgttg tgcttgtaat ttactgtgtt ttttattcgg ttttcgctat
1141 cgaactgtga aatggaaatg gatggagaag agttaatgaa tgatatggtc cttttgttca
1201 ttctcaaatt aatattattt gttttttctc ttatttgttg tgtgttgaat ttgaaaatat
1261 aagagatatg caaacatttt gttttgagta aaaatgtgtc aaatcgtggc ctctaatgac
1321 cgaagttaat atgaggagta aaacacttgt agttgtacca ttatgcttat tcactaggca
1381 acaaatatat tttcagacct agaaaagctg caaatgttac tgaatacaag tatgtcctct
1441 tgtgttttag acatttatga actttccttt atgtaatttt ccagaatcct tgtcagattc
1501 taatcattgc tttataatta tagttatact catggatttg tagttgagta tgaaaatatt
1561 ttttaatgca ttttatgact tgccaattga ttgacaacat gcatcaagct tggcactggc
1621 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
1681 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
1741 ccaacagttg cgcagcctga atggcgaatg ctagagcagc ttgagcttgg atcagattgt
1801 cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc gggtaaacct
1861 aagagaaaag agcgtttatt agaataacgg atatttaaaa gggcgtgaaa aggtttatcc
1921 gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg
1981 atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct
2041 gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc
2101 tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg
2161 agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc
2221 accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag
2281 ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt
2341 gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc
2401 acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc
2461 ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc
2521 gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag
2581 gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc
2641 gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg
2701 cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc
2761 accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg
2821 gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg
2881 acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt
2941 tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg
3001 atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc
3061 taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga
3121 tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa
3181 ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact
3241 cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc
3301 ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg
3361 cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc
3421 ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag
3481 cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt
3541 cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat
3601 cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat
3661 cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga
3721 acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact
3781 catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc
3841 cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc
3901 atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg
3961 gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag
4021 taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga
4081 aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg
4141 ttggccaggc gtaagcggct gggttgtctg ccggccctgc aatggcactg gaacccccaa
4201 gcccgaggaa tcggcgtgac ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc
4261 tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg
4321 aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat
4381 cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc
4441 aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca
4501 tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct
4561 acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt
4621 gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc
4681 gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca
4741 agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc
4801 ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg
4861 tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg
4921 ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag
4981 gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg
5041 gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt
5101 tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg
5161 tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc
5221 aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg
5281 gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa
5341 aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc
5401 ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga
5461 ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa
5521 ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc
5581 aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta
5641 tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg
5701 gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg
5761 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
5821 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
5881 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
5941 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
6001 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
6061 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
6121 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
6181 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
6241 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
6301 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
6361 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
6421 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
6481 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
6541 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
6601 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
6661 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
6721 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
6781 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
6841 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcattctagg tactaaaaca
6901 attcatccag taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt
6961 caaaaaatag ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga
7021 aggcaatgtc ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta
7081 ctttgccatc tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt
7141 cctcttcggg cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag
7201 tgtcttcttc ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca
7261 attcggctaa gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt
7321 gaaagagcct gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt
7381 catactcttc cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat
7441 catgccgttc aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca
7501 tgtccttttc ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta
7561 aatataggtt ttcattttct cccaccagct tatatacctt agcaggagac attccttccg
7621 tatcttttac gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt
7681 tagccattta ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa
7741 ttataacaag acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa
7801 aacagctttt tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat
7861 tttgaaaccg cggtgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac
7921 cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc
7981 atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg
8041 actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt
8101 tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat
8161 aacacattgc ggacgttttt aatgtactga attaacgccg aattaattcg ggggatctgg
8221 attttagtac tggattttgg ttttaggaat tagaaatttt attgatagaa gtattttaca
8281 aatacaaata catactaagg gtttcttata tgctcaacac atgagcgaaa ccctatagga
8341 accctaattc ccttatctgg gaactactca cacattatta tggagaaact cgagcttgtc
8401 gatcgacaga tccggtcggc atctactcta tttctttgcc ctcggacgag tgctggggcg
8461 tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct
8521 gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg
8581 accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc
8641 aagaccaatg cggagcatat acgcccggag tcgtggcgat cctgcaagct ccggatgcct
8701 ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat
8761 gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt
8821 tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg
8881 gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg cgacggacgc
8941 actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag caatcgcgca
9001 tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg ggccgaaccc
9061 gctcgtctgg ctaagatcgg ccgcagcgat cgcatccata gcctccgcga ccggttgtag
9121 aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg cacggcggga
9181 gatgcaatag gtcaggctct cgctaaactc cccaatgtca agcacttccg gaatcgggag
9241 cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat cggcgcagct
9301 atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac gagattcttc
9361 gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca gaaacttctc
9421 gacagacgtc gcggtgagtt caggcttttt catatctcat tgccccccgg gatctgcgaa
9481 agctcgagag agatagattt gtagagagag actggtgatt tcagcgtgtc ctctccaaat
9541 gaaatgaact tccttatata gaggaaggtc ttgcgaagga tagtgggatt gtgcgtcatc
9601 ccttacgtca gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc
9661 ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga
9721 ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc
9781 cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt
9841 tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc
9901 tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttatcac atcaatccac
9961 ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg
10021 tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca
10081 atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat
10141 agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat
10201 agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg
10261 ctccaccatg ttggcaagct gctctagcca atacgcaaac cgcctctccc cgcgcgttgg
10321 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
10381 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
10441 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
10501 gacatgatta c
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