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基本信息

質(zhì)粒圖譜和多克隆位點(diǎn)信息

注意：

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如要使用本公司保存的母源載體時，本公司不能保證所有母源載體完全符合客戶預(yù)期，也不承擔(dān)由此產(chǎn)生的任何責(zé)任。

載體描述

pCMV-C-mCherry為哺乳動物細(xì)胞表達(dá)質(zhì)粒，用于表達(dá)C端含mCherry (DsRed的突變體，一種非常明亮的紅色熒光蛋白)標(biāo)簽的融合蛋白。該質(zhì)粒含有CMV啟動子，可以高效啟動目的蛋白在細(xì)胞中的表達(dá)。在多克隆位點(diǎn)的后面有一個mCherry的完整編碼序列，因此在多克隆位點(diǎn)根據(jù)閱讀框插入目的基因就可以表達(dá)C端含有mCherry標(biāo)簽的融合蛋白。利用mCherry的熒光特性可以比較容易地觀察融合蛋白的表達(dá)水平和細(xì)胞內(nèi)定位，也可以利用mCherry抗體來檢測或免疫沉淀融合蛋白。mCherry與GFP沒有序列同源性，不能使用GFP抗體檢測mCherry。該質(zhì)粒為卡那霉素抗性。轉(zhuǎn)染細(xì)胞后，可以使用G418篩選穩(wěn)定表達(dá)目的蛋白的細(xì)胞株。

載體序列

ORIGIN

        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga

       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg

      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg

      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca

      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc

      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc

      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc

      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa

      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag

      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta

      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc

      661 gcggtggcgg ccgctctagc ccgggcggat ccaagcttct gcaggaattc gatatcgtcg

      721 acagatctct cgagtctaga atggtgagca agggcgagga ggataacatg gccatcatca

      781 aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga

      841 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga

      901 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct

      961 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg

     1021 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc

     1081 aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact

     1141 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc

     1201 ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg

     1261 acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc

     1321 tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca

     1381 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc

     1441 tgtacaagta ggggcccggt accttaatta attaaggtac caggtaagtg tacccaattc

     1501 gccctatagt gagtcgtatt acaattcact cgatcgccct tcccaacagt tgcgcagcct

     1561 gaatggcgaa tggagatcca atttttaagt gtataatgtg ttaaactact gattctaatt

     1621 gtttgtgtat tttagattca cagtcccaag gctcatttca ggcccctcag tcctcacagt

     1681 ctgttcatga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac

     1741 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg

     1801 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa

     1861 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc

     1921 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc

     1981 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga

     2041 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc

     2101 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc

     2161 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag

     2221 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa

     2281 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac

     2341 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa

     2401 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat

     2461 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagaatc ctgaggcgga

     2521 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca

     2581 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca

     2641 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc

     2701 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc

     2761 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta

     2821 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga

     2881 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc

     2941 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga

     3001 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct

     3061 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac

     3121 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct

     3181 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt

     3241 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt

     3301 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt

     3361 cgatcaggat gatctggacg aagaacatca ggggctcgcg ccagccgaac tgttcgccag

     3421 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt

     3481 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg

     3541 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagaacttgg

     3601 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg

     3661 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg

     3721 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat

     3781 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg

     3841 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag

     3901 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt

     3961 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc

     4021 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc

     4081 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata

     4141 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg

     4201 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg

     4261 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt

     4321 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg

     4381 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata

     4441 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca

     4501 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag

     4561 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc

     4621 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga

     4681 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg

     4741 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac

     4801 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg

     4861 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg

     4921 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct

     4981 gtggataacc gtattaccgc c

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