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基本信息
載體類型: | 哺乳動(dòng)物細(xì)胞表達(dá)載體 |
載體抗性: | Kan |
載體標(biāo)簽: | / |
啟動(dòng)子: | CMV |
篩選標(biāo)記: | Neo/G418 |
表達(dá)水平: | 高拷貝 |
載體大小: | 2999bp |
克隆方法: | 多克隆位點(diǎn),限制性內(nèi)切酶 |
5' 測(cè)序引物: | CMV-F:5’-CGCAAATGGGCGGTAGGC-3’ |
3' 測(cè)序引物: | BGH R:5’-TAGAAGGCACAGTCGAGG-3’ |
備注: | 如有侵權(quán)請(qǐng)產(chǎn)權(quán)持有人盡快聯(lián)系法務(wù)15927229196 |
質(zhì)粒圖譜和多克隆位點(diǎn)信息
注意:
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如要使用本公司保存的母源載體時(shí),本公司不能保證所有母源載體完全符合客戶預(yù)期,也不承擔(dān)由此產(chǎn)生的任何責(zé)任。
載體序列
ORIGIN
1 gactcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
61 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
121 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
181 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
241 ctatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
301 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
361 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
421 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
481 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
541 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
601 ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga
661 aattaatacg actcactata gggagaccca agctggctag cgtttaaact taagcttggt
721 accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcca gcacagtggc
781 ggccgctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
841 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
901 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
961 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
1021 gcaggcatgc tggggatgcg gtgggctcta tggcttctac tgggcggttt tatggacagc
1081 aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt
1141 aaactggatg gctttctcgc cgccaaggat ctgatggcgc aggggatcaa gctctgatca
1201 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
1261 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
1321 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
1381 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac
1441 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
1501 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
1561 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
1621 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
1681 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
1741 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
1801 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
1861 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
1921 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
1981 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga attattaacg cttacaattt
2041 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacaggtggc
2101 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
2161 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatagca cgtgctaaaa
2221 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
2281 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
2341 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
2401 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
2461 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
2521 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
2581 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
2641 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
2701 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
2761 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
2821 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
2881 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
2941 agcaacgcgg cctttttacg gttcctgggc ttttgctggc cttttgctca catgttctt
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