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基本信息

載體類型: 

哺乳動物細胞表達載體;紅色熒光報告載體 

載體抗性: 

Kan 

載體標簽: 

DsRed-Express,紅色熒光蛋白 

篩選標記: 

新霉素 

啟動子: 

CMV 

表達水平: 

高拷貝 

載體大小: 

4691bp 

克隆方法: 

多克隆位點,限制性內(nèi)切酶 

5' 測序引物: 

CMV-F: 5’-CGCAAATGGGCGGTAGGCGTG-3’ 

3' 測序引物 

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備注:

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質(zhì)粒圖譜和多克隆位點信息 

注意:

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如要使用本公司保存的母源載體時,本公司不能保證所有母源載體完全符合客戶預期,也不承擔由此產(chǎn)生的任何責任。  

 

載體序列

ORIGIN

        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg

       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt

      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca

      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc

      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta

      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac

      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg

      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg

      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt

      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta

      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg

      661 gatccaccgg tcgccaccat ggacaacacc gaggacgtca tcaaggagtt catgcagttc

      721 aaggtgcgca tggagggctc cgtgaacggc cactacttcg agatcgaggg cgagggcgag

      781 ggcaagccct acgagggcac ccagaccgcc aagctgcagg tgaccaaggg cggccccctg

      841 cccttcgcct gggacatcct gtccccccag ttccagtacg gctccaaggc ctacgtgaag

      901 caccccgccg acatccccga ctacatgaag ctgtccttcc ccgagggctt cacctgggag

      961 cgctccatga acttcgagga cggcggcgtg gtggaggtgc agcaggactc ctccctgcag

     1021 gacggcacct tcatctacaa ggtgaagttc aagggcgtga acttccccgc cgacggcccc

     1081 gtaatgcaga agaagactgc cggctgggag ccctccaccg agaagctgta cccccaggac

     1141 ggcgtgctga agggcgagat ctcccacgcc ctgaagctga aggacggcgg ccactacacc

     1201 tgcgacttca agaccgtgta caaggccaag aagcccgtgc agctgcccgg caaccactac

     1261 gtggactcca agctggacat caccaaccac aacgaggact acaccgtggt ggagcagtac

     1321 gagcacgccg aggcccgcca ctccggctcc cagtagagcg gccgcgactc tagatcataa

     1381 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc

     1441 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata

     1501 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc

     1561 attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt

     1621 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag

     1681 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt

     1741 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga

     1801 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg

     1861 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct

     1921 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc

     1981 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt

     2041 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct

     2101 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga

     2161 taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga

     2221 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa

     2281 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca

     2341 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc

     2401 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt

     2461 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag

     2521 gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg

     2581 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg

     2641 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg

     2701 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat

     2761 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca

     2821 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg

     2881 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat

     2941 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa

     3001 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg

     3061 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg

     3121 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg

     3181 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat

     3241 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac

     3301 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc

     3361 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc

     3421 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg

     3481 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt

     3541 tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa

     3601 cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc

     3661 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt

     3721 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag

     3781 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat

     3841 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc

     3901 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag

     3961 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct

     4021 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac

     4081 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc

     4141 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg

     4201 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt

     4261 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt

     4321 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc

     4381 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca

     4441 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata

     4501 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg

     4561 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct

     4621 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta

     4681 ccgccatgca t

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